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DOT Drug Testing

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​DOT Urine Drug Testing

Employees who are designated in DOT regulations as safety-sensitive are subject to DOT drug and alcohol testing.

DOT drug tests are conducted using urine specimens only for the following drugs/metabolites:

  • Marijuana metabolites/THC,
  • Cocaine Metabolites
  • Amphetamines (including methamphetamines, MDMA)
  • Opiates (including codeine, heroin, morphine)
  • Phencyclidine (PCP)


The following are defined as safety-sensitive function and are subject to testing:

Federal Aviation Administration (FAA): Flight crews, flight attendants, flight instructors, air traffic controllers at facilities not operated by the FAA or under contract to the U.S. military, aircraft dispatchers, aircraft maintenance or preventive maintenance personnel, ground security coordinators and aviation screeners.


Commercial Motor Carriers (FMCSA): Commercial Drivers License (CDL) holders who operate Commercial Motor Vehicle, 26, 001 lbs. gvwr. or greater, or operate a vehicle that carries 16 passengers or more including the driver, or required to display a DOT placard in the Transportation of hazardous materials.


Federal Railroad Adminstration (FRA): Engine and train, signal service, train dispatchers.


Federal Transit Authority (FTA): Vehicle operators, controllers, mechanics and armed security.


US Coast Guard (USCG): Crewmembers operating a commercial vessel.


Pipeline and Hazardous Materials Safety Admin: Operations, maintenance and emergency response.








​​​​​​​​​​​DOT TESTING

  • Pre-Employment: As a new hire, you are required to submit to a drug test. Only after your employer receives a negative drug test result may you begin performing safety-sensitive functions.
  • Random:Employee are subject to random drug & alcohol testing.  Alcohol testing is administered just prior to, during or just after performing safety-sensitive functions.
  • Post-Accident: When an employee is involved in an accident that meet certain criteria of the DOT agency, a post-accident test will be required.  The employee will then have to take a drug and alcohol test.
  • Reasonable Suspicion:Employees are required to submit to any test (whether drug and alcohol or both) that a supervisor requests based on reasonable suspicion.  Reasonable suspicion means that one or more trained supervisors reasonably believes or suspects that an employee is under the influence of drugs or alcohol.
  • Return-to-Duty: If an employee violated the prohibited drug & alcohol rules, he/she is required to take a drug and/or alcohol test before returning to safety-sensitive functions for any DOT regulated employer.  The employee is subject to unannounced follow-up testing at least 6 times in the first 12 months following his/her return to active safety-sensitive service.  Return-to-duty tests must be conducted under direct observation.
  • Follow-up: The amount of follow-up testing an employee receives is determined by a Substance Abuse Professional (SAP) and may continue for up to 5 years.  This means the SAP will determine how many times the employee will be tested and for how long and for what substance.  As the employer you are responsible for ensuring that the follow-up testing is conducted and completed.